Homogentisate 1,2-dioxygenase Antibody / HGD

The HGD gene encodes homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q93099

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids D374-N445) was used as the immunogen for the Homogentisate 1,2-dioxygenase antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

2F11E1

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Homogentisate 1,2-dioxygenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Homogentisate 1,2-dioxygenase antibody is available for research use only.

Storage Conditions

After reconstitution, the Homogentisate 1,2-dioxygenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Homogentisate 1,2-dioxygenase antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human liver cancer tissue with Homogentisate 1,2-dioxygenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.