H1F0 Antibody / Histone H1.0

H1 histone family, member 0 is a member of the histone family of nuclear proteins which are a component of chromatin. In humans, this protein is encoded by the H1F0 gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P07305

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Recombinant human protein (amino acids K20-K159) was used as the immunogen for the H1F0 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

5I3E6

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the H1F0 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This H1F0 antibody is available for research use only.

Storage Conditions

After reconstitution, the H1F0 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the H1F0 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE Hodgkin's lymphoma tissue with H1F0 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.