Uridylate-specific endoribonuclease Antibody / PP11 / ENDOU

Endonuclease, poly (U) specific is a protein that in humans is encoded by the ENDOU gene. This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD) . The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P21128

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids K20-T386) was used as the immunogen for the Uridylate-specific endoribonuclease antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Uridylate-specific endoribonuclease antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Uridylate-specific endoribonuclease antibody is available for research use only.

Storage Conditions

After reconstitution, the Uridylate-specific endoribonuclease antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Uridylate-specific endoribonuclease antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human lung cancer tissue with Uridylate-specific endoribonuclease antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.