Cdc2 related protein kinase 2 Antibody / CDK2

CDK2, Cyclin-Dependent Kinase 2, is also known as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2) /cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2) . P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P24941

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids E81-L298) was used as the immunogen for the Cdc2 related protein kinase 2 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

6D5B5

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Cdc2 related protein kinase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cdc2 related protein kinase 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cdc2 related protein kinase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cdc2 related protein kinase 2 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human liver cancer tissue with Cdc2 related protein kinase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.