Sorbitol Dehydrogenase Antibody / SORD

Sorbitol dehydrogenase is an enzyme that in humans is encoded by the SORD gene. Sorbitol dehydrogenase (SORD) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD (+) cofactor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q00796

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human Sorbitol Dehydrogenase/SORD protein (amino acids N8-P357) was used as the immunogen for the Sorbitol Dehydrogenase antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

12B10G2

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Sorbitol Dehydrogenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Sorbitol Dehydrogenase antibody is available for research use only.

Storage Conditions

After reconstitution, the Sorbitol Dehydrogenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Sorbitol Dehydrogenase antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human liver cancer tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.