LARG Antibody / ARHGEF12

Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9NZN5

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein (amino acids T4-S254) was used as the immunogen for the LARG antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the LARG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LARG antibody is available for research use only.

Storage Conditions

After reconstitution, the LARG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LARG antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human HEL, 2) human K562 and 3) rat brain lysate with LARG antibody. Predicted molecular weight ~173 kDa but commonly observed at up to 220 kDa, possibly due to phosphorylation.