HGF-regulated tyrosine kinase substrate Antibody / HRS / HGS

Hepatocyte growth factor-regulated tyrosine kinase substrate is an enzyme that in humans is encoded by the HGS gene. It is mapped to 17q25.3. The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

O14964

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human HGS protein (amino acids R3-D777) was used as the immunogen for the HGF-regulated tyrosine kinase substrate antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

4B7E2

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the HGF-regulated tyrosine kinase substrate antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HGF-regulated tyrosine kinase substrate antibody is available for research use only.

Storage Conditions

After reconstitution, the HGF-regulated tyrosine kinase substrate antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HGF-regulated tyrosine kinase substrate antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human U-87 MG cells with HGF-regulated tyrosine kinase substrate antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.