FACL4 Antibody / ACSL4

Long-chain-fatty-acid--CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O60488

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human FACL4/ACSL4 protein (amino acids A36-E237) was used as the immunogen for the FACL4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the FACL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This FACL4 antibody is available for research use only.

Storage Conditions

After reconstitution, the FACL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the FACL4 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human U-2 OS, 2) human PC-3, 3) human HeLa, 4) human Caco-2, 5) human HEL, 6) human U-87 MG, 7) rat brain, 8) rat lung, 9) rat stomach, 10) rat PC-12, 11) mouse brain, 12) mouse lung, 13) mouse stomach and 14) mouse NIH 3T3 cell lysate with FACL4 antibody. Predicted molecular weight: ~80 kDa (long form), ~74 kDa (short form) .