P Glycoprotein Antibody / MDR1 / ABCB1

P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P08183

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human P Glycoprotein/ABCB1 protein (amino acids M1-E690) was used as the immunogen for the P Glycoprotein antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the P Glycoprotein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This P Glycoprotein antibody is available for research use only.

Storage Conditions

After reconstitution, the P Glycoprotein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the P Glycoprotein antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

Flow cytometry testing of human Caco-2 cells with P Glycoprotein antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= P Glycoprotein antibody.