Anti-CENP-A Antibody

Mouse Monoclonal Antibody specific to CENP-A

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Centromere autoantigen A, Centromere protein A, CENP-A

Gene Name

CENPA

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CENPA">CENPA</a>

UniProt

P49450

Accession Number

NP_001035891.1

Cellular Locus

Nucleus, Chromosome, centromere, kinetochore, Chromosome, centromere

Host

Mouse

Reactivity

Human

Immunogen

Synthetic peptide corresponding to a sequence within human CENP-A.

Target Antigen

Histone H3-like centromeric protein A

Target

CENP-A

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

DNA

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292). {PubMed:11756469, PubMed:14667408, PubMed:15282608, PubMed:15475964, PubMed:15702419, PubMed:17651496, PubMed:18072184, PubMed:19114591, PubMed:21478274, PubMed:23818633, PubMed:25556658, PubMed:26878239, PubMed:27499292, PubMed:7962047, PubMed:9024683, PubMed:20739937}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~18kDa is detected.Detection of CENP-A in lysate of U2OS cellsELISA: use at 1-10ug/mL with CENP-A on the solid phase. Positive control: U2OS cell lysate These are recommended concentrations; Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human CENP-A. Reactivity with other species has not been investigated.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Replicated chromosomes include two kinetochores that control chromosome segregation during mitosis. Centromere Protein-A (CENP-A), a histone H3-like protein, contains a C-terminal H3-like domain which is required for centromere localization of CENP-A. It is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H2A, H2B, and H4. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on chromosomes.