Anti-HNF4 α (phospho-Ser304) Antibody

Rabbit Polyclonal Antibody specific to HNF4 α (phospho-Ser304)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-HNF4 α (phospho-Ser304) Rabbit Polyclonal Antibody

Gene Name

HNF4A

Gene ID

600281

NCBI Gene ID

UniProt

P41235

Cellular Locus

Nucleus.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 304 (L-R-S(p)-Q-V) derived from human HNF4a and conjugated to KLH.

Target Antigen

Hepatocyte nuclear factor 4-α

Target

HNF4 α (phospho-Ser304)

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698). {UniProtKB:P49698, PubMed:30530698, PubMed:30597922}.

Additionnal Information

Immunoblotting: use at dilution of 1:1,000. A band of ~52kDa is detected. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their applications

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat HNF4alpha only when phosphorylated at serine 304.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Hepatocyte nuclear factor 4 alpha (HNF4alpha) is a nuclear transcription factor that binds DNA as a homodimer and controls the expression of several genes including hepatocyte nuclear factor 1, a transcripton factor that regulates expression of several hepatic genes. HNF4alpha has been implicated in development of liver, kidney, and intestines. Mutations in the HNF4alpha gene have been associated with monogenic autosomal-dominant non-insulin-dependent diabetes mellitus type 1. Alternative splicing of this gene results in multiple transcript variants.