PCSK9 (D374T) [Biotinylated]-LDLR Binding Assay Kit

PCSK9 (Proprotein convertase subtilisin/kexin type 9) functions as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain. The D374T mutation is associated with hypercholesterolemia; this form of PCSK9 is more potent at decreasing LDL uptake than wild-type PCSK9, most likely by increasing the binding affinity of PCSK9 for the LDLR.

96 reactionsCatalog #NameAmountStorage71211PCSK9 (D374T), Biotin-Labeled*10 µg-80°C71205LDLR*10 µg-20°C79742Streptavidin-HRP10 µl-20°C332983x PL-01 Assay Buffer50 ml-20°C79728Blocking Buffer 250 ml+4°C79670ELISA ECL Substrates A and B (2 components) 6 ml eachRoom Temp.7969996-well plate, white1+4°C* The concentration of protein is lot-specific and will be indicated on the tube containing the protein384 reactionsCatalog #NameAmountStorage71211PCSK9 (D374T), Biotin-Labeled*2 x 10 µg-80°CAvoid multiple freeze/ thaw cycles71205LDLR, FLAG-Tag*2 x 10 µg-80°C79742Streptavidin-HRP2 x 10 µl4°C332983x PL-01 Assay Buffer2 x 50 ml-20°C79728Blocking Buffer 22 x 50 ml4°C79670ELISA ECL Substrate A (translucent bottle) 2 x 6 mlRoom TempELISA ECL Substrate B (brown bottle) 2 x 6 mlRoom Temp78188384-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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