Human Keratinocyte Growth Factor Recombinant
Recombinant human Keratinocyte Growth Factor is a disulfide-linked monomeric protein consisting of 164 amino acid residues. Optimized DNA sequence encoding Human Keratinocyte Growth Factor mature chain was expressed inE. coli. Native human KGF is generated by the proteolytic removal of the signal peptide and propeptide. KGF migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Product Specifications
Background
KGF is a member of the family of fibroblast growth factors, it is secreted in large amounts by fibroblast-like stromal cells in epithelial tissues. The KGF receptor encodes a tyrosine kinase and is a member of the family of receptors binding aFGF and bFGF. Binding of KGF to its receptor is competed by aFGF and bFGF. Although KGF is a potent specific mitogen for many epithelial cells, it is not so for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF.
UniProt
P21781
Endotoxin
< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 96% by SDS-PAGE and HPLC
Format
Lyophilized protein
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.
Shipping Conditions
Gel Packs or Dry Ice
Storage Conditions
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Calculated Molecular Weight
19 kDa
Formulation
Lyophilized from a 0.2 µm filtered 20 mM Phosphate buffer, 1M NaCl, pH 8.0.
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