Human FLT3 Ligand Recombinant

Recombinant FLT-3 Ligand is a disulfide-linked monomeric protein consisting of 156 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human FLT-3 ligand mature chain was expressed inE. coli.

Product Specifications

Background

FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3.The human FLT-3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. In combination with SCF and IL-3, FLT-3 ligand can cause expansion of cells with the marker spectrum CD34 (+) CD38 (-) . Alone, FLT-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.

UniProt

P49771

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 97% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.