Human Fibroblast Growth Factor 21 Recombinant
Recombinant Fibroblast Growth Factor 21 is a disulfide-linked monomer protein consisting of 182 amino acid residues, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Fibroblast Growth Factor-21 mature chain was expressed inE. coli.
Product Specifications
Background
FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) . Activity requires the presence of KLB.
UniProt
Q9NSA1
Endotoxin
< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 96% by SDS-PAGE and HPLC
Format
Lyophilized protein
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Shipping Conditions
Gel Packs or Dry Ice
Storage Conditions
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Calculated Molecular Weight
20 kDa
Formulation
Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
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