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Human Exodus-2 Recombinant

Recombinant Exodus-2 is a disulfide-linked homodimeric protein consisting of 112 amino acid residues, and migrates as an approximately 12 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Exodus-2 mature chain was expressed inE. coli.

Product Specifications

Background

Exodus-2, which is a member of the family of CC- Chemokines, has a unique 36 (murine) or 37 (human) amino acid carboxyl-terminal extension not seen in other chemokines. Exodus-2 selectively stimulates the chemotaxis of adult T-lymphocytes and is expressed preferentially in lymph node tissue. Exodus-2 is critical for adult T-cell adhesion to high endothelial venules in lymph nodes, a rate-limiting step for T-cell trafficking through nodal tissue.

UniProt

O00585

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 95% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

12 kDa

Formulation

Lyophilized from 0.2 µm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween-80,5 mMglutamic acid, pH 4.5.

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