Human Eotaxin-3 (CCL26) Recombinant

Recombinant Eotaxin-3 is a disulfide-linked homodimeric protein consisting of 72 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Eotaxin-3 mature chain was expressed inE. coli.

Product Specifications

Background

Eotaxin belongs to the platelet factor-4 family of chemokines. The factor is being referred to also as Eotaxin-2 and has been renamed CCL24. Both eotaxin-2/CCL24 and eotaxin-3/CCL26 are CC chemokines and functional ligands for CC chemokine receptor 3 (CCR3), which is preferentially expressed on eosinophils. Only 34% of the amino acids in eotaxin-2/CCL24 and eotaxin-3/CCL26 are identical, but these two chemokines have a common characteristic feature in terms of chemotaxis for eosinophils via the CCR3.

UniProt

Q9Y258

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 97% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.