Human Eotaxin-1 (CCL11) Recombinant
Recombinant Eotaxin-1 is a disulfide-linked homodimeric protein consisting of 75 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Eotaxin-1 mature chain was expressed inE. coli.
Product Specifications
Background
Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophilsin vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils .
UniProt
P51671
Endotoxin
< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 97% by SDS-PAGE and HPLC
Format
Lyophilized protein
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Shipping Conditions
Gel Packs or Dry Ice
Storage Conditions
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Calculated Molecular Weight
9 kDa
Formulation
Lyophilized from 0.2 µm filtered solution (1 mg/ml) in PBS, pH 7.4.
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