Human EMAP-II Recombinant
Recombinant EMAP-II is a disulfide-linked monomer protein consisting of 167 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human EMAP-II mature chain was expressed inE. coli.
Product Specifications
Background
EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase-8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells.
UniProt
Q12904
Endotoxin
< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 95% by SDS-PAGE and HPLC
Format
Lyophilized protein
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Shipping Conditions
Gel Packs or Dry Ice
Storage Conditions
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Calculated Molecular Weight
18 kDa
Formulation
Lyophilized from 0.2 µm filtered 20 mM phosphate buffer, 130 mM NaCl solution, pH 7.5.
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