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Human Endocrine Gland Vascular Endothelial Growth Factor Recombinant

Recombinant EG-VEGF is a disulfide-linked monomer protein consisting of 87 amino acid residues, and migrates as an approximately 10 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human EG-VEGF mature chain was expressed inE. coli.

Product Specifications

Background

This factor is identical with prokineticin-1 and is a member of the AVIT protein family. Expression of human EG-VEGF messenger RNA is restricted to the steroidogenic glands, ovary, testis, adrenal and placenta and is often complementary to the expression of VEGF. EG-VEGF has been identified as a mitogen specific for the endothelium of steroidogenic glands. EG-VEGF resembles VEGF in that it causes extensive angiogenesis and cyst formation when delivered in the ovary. EG-VEGF differs from VEGF in that it does not promote angiogenesis in the cornea or skeletal muscle. Two receptors (the G-protein-coupled receptor ZAQ and the G-protein-coupled receptor I5E) have been characterized, these receptors are expressed in gastrointestinal organs, endocrine glands and other tissues.

UniProt

P58294

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 98% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Calculated Molecular Weight

10 kDa

Formulation

Lyophilized from 0.2 µm filtered PBS solution.

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