Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity

Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. Papain-Like Protease (PLPro), also known as PLP, plays an essential role in polypeptide processing during virus replication. PLProis also proposed to be a key enzyme in the sustained pathogenesis of SARS-CoV-2. PLP acts as a deubiquitinase that removes ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate antiviral immune responses. As a result, PLPro is an important potential target for antiviral drugs that may inhibit viral replication and simultaneously weaken dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.

96 ReactionsCatalogNumberComponentAmountStorage100735Recombinant Papain-like Protease, PLPro2 µg-80°CAvoidfreeze/thawcycles!79997PLPro Substrate (5 mM) 25 µl-80°C78039PLPro Assay Buffer25 ml-20°C10 mM GRL0617 (10 mM) 20 µl-80°CDithiothreitol (DTT; 0.5 M) 200 µl-20°C79685Black, low binding microtiter plate1RoomTempPlate sealing film1384 ReactionsCatalogNumberComponentAmountStorage100735Recombinant Papain-like Protease, PLPro2 µg-80°CAvoidfreeze/thawcycles!79997PLPro Substrate (5 mM) 50 µl-80°C78039PLPro Assay Buffer25 ml-20°CGRL0617 (10 mM) 20 µl-80°CDithiothreitol (DTT; 0.5 M) 200 µl-20°C79961Black, low binding microtiter plate1RoomTempPlate sealing film2