IL-10 (Human) Colorimetric ELISA Detection Kit

Interleukin-10 is a key immunoregulator during various infections that acts as an anti-inflammatory cytokine by inhibiting the activities of Th1, macrophage and NK cells. The IL-10 (Human) Colorimetric ELISA Detection Kit is designed for detecting and quantifying human interleukin-10 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capturing antibody is coated on a 96-well plate. Next, samples containing IL-10 are incubated on the coated plate followed by detecting the captured IL-10 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Product Specifications

CAS Number

7732-18-5

UniProt

P22301

Format

96 Reactions:CatalogNumberComponentAmountStorageInterleukin-10 capturing antibody (1 mg/ml) 10 µl-80°CAvoidfreeze/thawcycles!Interleukin-10 detection antibody, biotinylated5 µl-80°CHuman IL-10 standard (1 μg/ml) 20 µl-80°C80611Streptavidin-HRP5 µl+4°C79743Blocking Buffer 350 ml+4°C79651Colorimetric HRP substrate10 ml+4°C79964Transparent 96-well microplate1+4°C5x 96 Reactions:CatalogNumberComponentAmountStorageInterleukin-10 capturing antibody (1 mg/ml) 50 µl-80°CAvoidfreeze/thawcycles!Interleukin-10 detection antibody, biotinylated25 µl-80°CHuman IL-10 standard (1 μg/ml) 100 µl-80°C80611Streptavidin-HRP25 µl+4°C79743Blocking Buffer 3250 ml+4°C79651Colorimetric HRP substrate50 ml+4°C79898Transparent 96-well microplate5x 1+4°C