IFN-γ (Human) Colorimetric ELISA Detection Kit

IFN-γ is a cytokine that is primarily secreted by activated T cells and natural killer (NK) cells and plays an important role in activating innate/adaptive immunity. It also has been recognized as an indicator of T cell activation suggesting IFN-γ activation may be a prerequisite for CAR-T cell activity. The IFN-γ Colorimetric ELISA Detection Kit is designed for detecting and quantifying human interferon-γ in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IFN-γ are incubated on the coated plate followed by detecting the captured IFN-γ with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.This product has beencited 3 times.

Product Specifications

CAS Number

7732-18-5

UniProt

P01579

Format

96 Reactions:Catalog #ComponentAmountStorageInterferon-γ capture antibody10 µl-80°CAvoidfreeze/thawcycles!Interferon-γ detection antibody, biotinylated5 µl-80°CHuman IFN-γ standard (1 µg/ml) 20 µl-80°C80611Streptavidin-HRP5 µl+4°C79743Blocking Buffer 350 ml+4°C79651Colorimetric HRP substrate10 ml+4°C79964Transparent 96-well microplate1+4°C5 x 96 Reactions:Catalog #ComponentAmountStorageInterferon-γ capture antibody5 x 10 µl-80°CAvoidfreeze/thawcycles!Interferon-γ detection antibody, biotinylated5 x 5 µl-80°CHuman IFN-γ standard (1 µg/ml) 5 x 20 µl-80°C80611Streptavidin-HRP5 x 5 µl+4°C79743Blocking Buffer 35 x 50 ml+4°C79651Colorimetric HRP substrate5 x 10 ml+4°C79964Transparent 96-well microplate5 x 1+4°C