IL-2 (Human) Colorimetric ELISA Detection Kit

The cytokine protein Interleukin-2 is an important factor for the maintenance of CD4+ regulatory T cells. It plays a critical role in the differentiation of CD4+ T cells into a variety of subsets as well as promotes CD8+ T-cell and NK cell cytotoxicity. The IL-2 (Human) Colorimetric Detection Kit is designed for detecting and quantifying human interleukin-2 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-2 are incubated on the coated plate followed by detecting the captured IL-2 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Product Specifications

CAS Number

7732-18-5

UniProt

P60568

Format

96 Reactions:CatalogNumberComponentAmountStorageInterleukin-2 capture antibody20 µl-80°C (Avoid freeze/ thaw cycles!) Interleukin-2 detection antibody, biotinylated5 µl-80°CHuman IL-2 standard (1 µg/ml) 20 µl-80°C80611Streptavidin-HRP5 µl+4°C79743Blocking Buffer 350 ml+4°C79651Colorimetric HRP substrate10 ml+4°CTransparent 96-well microplate1+4°C5 x 96 Reactions:CatalogNumberComponentAmountStorageInterleukin-2 capture antibody5 x 20 µl-80°C (Avoid freeze/ thaw cycles!) Interleukin-2 detection antibody, biotinylated5 x 5 µl-80°CHuman IL-2 standard (1 µg/ml) 40 µl-80°C80611Streptavidin-HRP5 x 5 µl+4°C79743Blocking Buffer 35 x 50 ml+4°C79651Colorimetric HRP substrate5 x 10 ml+4°CTransparent 96-well microplate5 x 1+4°C