RANK:RANKL TR-FRET Assay
The RANK:RANKL TR-FRET Assay is designed to measure the inhibition of RANK binding to RANKL. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for one hour. Then, the fluorescence intensity is measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against RANK:RANKL? Check out ourImmunotherapy Biochemical Screening Services.
Product Specifications
UniProt
RANK: Q9Y6Q6; RANKL: O14788
Format
96 Reactions:Catalog NumberComponents for 96 ReactionsAmountStorage70822RANK, Fc fusion (IgG1), Biotin Labeled (Human) 5 µg-80°CAvoid freeze/thaw cycles!71051RANKL-His2 µg-80°C30017Anti-His Tb Donor3 x 10 µl-20°CDye-labeled Acceptor3 x 10 µl-20°C793113x Immuno Buffer 14 ml-20°CWhite, 96-well half-area microtiter plate1Room Temp.384 Reactions:Catalog NumberComponents for 384 ReactionsAmountStorage70822RANK, biotinylated5 µg-80°CAvoid freeze/thaw cycles!71051RANKL-His2 µg-80°C30017Anti-His Tb Donor2 x 10 µl-20°CDye-labeled Acceptor2 x 10 µl-20°C793113x Immuno Buffer 14 ml-20°CWhite, non-binding, low volume, 384-well microtiter plate1Room Temp.
Shipping Conditions
-80°C
Storage Conditions
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
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