NAMPT Inhibitor Screening Assay Kit

The NAMPT Inhibitor Screening Assay Kit is a 96-well or 384-well fluorogenic assay designed to measure the activity of NAMPT (nicotinamide phosphoribosyltransferase) for screening and profiling applications. The kit contains enough purified NAMPT enzyme, NAMPT Assay Buffer, NAMPT Dilution Buffer, ATP, Nicotinamide, PRPP and ethanol for 100 or 400 enzyme reactions.Figure 1 – Illustration of assay principle used in the NAMPT Inhibitor Screening Assay Kit.This assay detects NAMPT activity through a series of three coupled reactions. NAMPT catalyzes the conversion of nicotinamide (NAM) and PRPP into nicotinamide mononucleotide (NMN) . NMN is then converted by NMNAT into NAD+, which is subsequently reduced to NADH by alcohol dehydrogenase (ADH) in the presence of ethanol. NADH emits fluorescence at 460 nm upon excitation at 340 nm. The intensity of this fluorescence correlates with the amount of NADH produced in the final step and it is directly proportional to the activity of NAMPT in the initial reaction.Need us to run inhibitor screens or profile your compounds against NAMPT? Check out ourImmunotherapy Biochemical Screening Services.This product has beencited 2 times.