PCSK9 (D374T), Biotin-labeled, HiP™ Recombinant
Recombinant human PCSK9 (Proprotein convertase subtilisin/kexin type 9), encompassing amino acids 31-692 (end) . The protein contains mutation of interest D374T, and a C-terminal His-tag (6xHis) followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.HiP™indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
Product Specifications
Background
UniProt
Q8NBP7
Tag
C-terminal His, Avi-tags
Label
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Purity
≥ 90%
Format
Aqueous buffer solution
Shipping Conditions
-80°C
Storage Conditions
At least 6 months at –80°C.
Calculated Molecular Weight
Pro: 14 kDa + glycans; Mature: 60 kDa + glycans
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Aggregation
< 10%
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
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