DNMT1 Chemiluminescent Assay Kit

The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against DNMT1? Check out ourMethyltransferase Screening Services.This product has beencited 3 times.

Product Specifications

UniProt

P26358

Applications

Useful for screening enzyme inhibitors.

Format

CatalogNumberComponentAmountStorage51101DNMT12 x 10 μg-80°CAvoid freeze/thaw cycles!52120400 μM S-adenosylmethionine2 x 250 μl-80°CAnti-5-methylcytosine antibody25 µl-80°C52130HSecondary HRP-labeled antibody 110 µl-80°C522004x DNMT assay buffer 1**Add 260 μl of 0.5 M DTT before use.5 ml-20°C52100Blocking buffer 450 ml+4°CHRP chemiluminescent substrate A (translucent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°CPlate precoated with DNMT1 substrate1+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.