PRMT4 Chemiluminescent Assay Kit

The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.This product has beencited 3 times.

Product Specifications

Background

PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.

UniProt

Q86X55

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

Catalog #ComponentAmountStorage51047PRMT4/CARM1 enzyme20 µg-80°C (Avoid freeze/ thaw cycles!) 5212020 µM S-adenosylmethionine250 µl-80°C52140IPrimary antibody 9100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C1% BSA50 µl-80°C521914x HMT assay buffer 5*3 ml-20°C52100Blocking Buffer 450 ml+4°CHRP chemiluminescent substrate (2 components) 6 ml each+4°C96-well plate precoated with PRMT4 substrate1 plate+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.