PRMT4 Chemiluminescent Assay Kit
The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.This product has beencited 3 times.
Product Specifications
Background
PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.
UniProt
Q86X55
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Format
Shipping Conditions
-80°C
Storage Conditions
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt
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