SUV39H1 Chemiluminescent Assay Kit

TheSUV39H1 Chemiluminescent Assay Kitis designed to measure SUV39H1 activity for screening and profiling applications. The key to theSUV39H1 Chemiluminescent Assay Kitis a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Product Specifications

UniProt

O43463

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

Catalog #ComponentAmountStorage51070SUV39H1 human enzyme*10 µg-80°CAvoid freeze/thaw cycles!52120100 µM S-adenosylmethionine250 µl-80°C52140APrimary antibody 1100 µl-80°C52130HSecondary HRP-labeled antibody 110 µl-80°C521604x HMT assay buffer 13 ml-20°C79556Blocking buffer 150 ml+4°C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom Temp.ELISA ECL substrate B (brown bottle) 6 mlRoom Temp.96-well strip plate precoated with histone substrate1 plate+4°C*The concentration of SUV39H1 is lot-specific and will be indicated on the tube containing the enzyme.

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.