PRMT3 Chemiluminescent Assay Kit

The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3 activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.This product has beencited 1 time.

Product Specifications

UniProt

Q8WUV3

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage51043PRMT3 human recombinant enzyme10 µg-80°CAvoid freeze/thaw cycles!5212052120 20 μM S-adenosylmethionine*250 µl-80°C52150Primary antibody 4100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C521704x HMT assay buffer 23 ml-20°C52100Blocking buffer 450 ml+4°CHRP chemiluminescent A (transparent bottle) 6 ml+4°CHRP chemiluminescent B (brown bottle) 6 ml+4°C96-well plate precoated with histonesubstrate1 plate+4°C*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to inhibitors.