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PRMT3 Chemiluminescent Assay Kit

Selected Variant

52005L

Size: 96 Reactions

The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3 activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.This product has beencited 1 time.

Product Specifications

UniProt

Q8WUV3

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage51043PRMT3 human recombinant enzyme10 µg-80°CAvoid freeze/thaw cycles!5212052120 20 μM S-adenosylmethionine*250 µl-80°C52150Primary antibody 4100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C521704x HMT assay buffer 23 ml-20°C52100Blocking buffer 450 ml+4°CHRP chemiluminescent A (transparent bottle) 6 ml+4°CHRP chemiluminescent B (brown bottle) 6 ml+4°C96-well plate precoated with histonesubstrate1 plate+4°C*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to inhibitors.

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt when stored as directed.

Contraindications

DMSO > 1%, strong acids or bases, ionic detergents, high salt

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