PRMT1 Chemiluminescent Assay Kit

The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PRMT1? Check out ourMethyltransferase Screening Services.This product has beencited 6 times.

Product Specifications

Background

PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

UniProt

Q99873

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage51040PRMT1 human recombinant enzyme10 µg-80°CAvoid freeze/ thaw cycles!5212020 µM S-adenosylmethionine*250 µl-80°C52150Primary antibody 4100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C521704x HMT assay buffer 23 ml-20°C52100Blocking buffer 450 ml+4°C79670ELISA ECL Substrate A (transparent bottle) 6 mlRT79670ELISA ECL Substrate B (brown bottle) 6 mlRT96-well plate precoated with histone substrate1 plate+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.