PRMT1 Chemiluminescent Assay Kit
The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PRMT1? Check out ourMethyltransferase Screening Services.This product has beencited 6 times.
Product Specifications
Background
PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
UniProt
Q99873
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Format
Shipping Conditions
-80°C
Storage Conditions
Notes
* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt
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