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PRMT1 Chemiluminescent Assay Kit

Selected Variant

52004L

Size: 96 Reactions

The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PRMT1? Check out ourMethyltransferase Screening Services.This product has beencited 6 times.

Product Specifications

Background

PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

UniProt

Q99873

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage51040PRMT1 human recombinant enzyme10 µg-80°CAvoid freeze/ thaw cycles!5212020 µM S-adenosylmethionine*250 µl-80°C52150Primary antibody 4100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C521704x HMT assay buffer 23 ml-20°C52100Blocking buffer 450 ml+4°C79670ELISA ECL Substrate A (transparent bottle) 6 mlRT79670ELISA ECL Substrate B (brown bottle) 6 mlRT96-well plate precoated with histone substrate1 plate+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Notes

* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.

Contraindications

DMSO > 1%, strong acids or bases, ionic detergents, high salt

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