PRMT1 Chemiluminescent Assay Kit
Selected Variant
52004L
Size: 96 Reactions
The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PRMT1? Check out ourMethyltransferase Screening Services.This product has beencited 6 times.
Product Specifications
Background
PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
UniProt
Q99873
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Format
Shipping Conditions
-80°C
Storage Conditions
Notes
* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt
Price
Frequently Asked Questions
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