PRMT5 Chemiluminescent Assay Kit

The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PRMT5? Check out ourMethyltransferase Screening ServicesorDNA Replication & Repair Screening Services.

Product Specifications

Background

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

UniProt

O14744

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentsAmountStorage51045PRMT5/MEP50 human enzyme20 µg-80°CAvoidfreeze/thawcycles!52120100 µM S-adenosylmethionine250 µl-80°C52150-3Primary antibody 4-3100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C4x PRMT5 assay buffer (add DTT before experiment) 3 ml-20°C79556Blocking buffer50 ml+4°CHRP chemiluminescent substrate A (transparent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°C96-well plate precoated with histone substrate1+4°C*The concentration of PRMT5/MEP50 is lot-specific and will be indicated on the tube containing the enzyme.

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Notes

Since May of 2017, this kit has been improved with a more specific antibody and reformulated buffers. The previous version of PRMT kit #52002L can still be purchased upon special request.