SetDB1 Chemiluminescent Assay Kit

The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The SetDB1 Chemiluminescent Assay Kit comes in a convenient 96-well or 384-well format, precoated with histone H3 peptide substrate, an antibody against the methylated lysine 9 residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SetDB1 enzyme (amino acids 510-1290) for 96 or 384 enzyme reactions. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence readerThis product has beencited 1 time.

Product Specifications

Background

SetDB1 (Histone-lysine N-methyltransferase SETDB1) is a methyltransferase that reversibly catalyzes the di and trimethylation of histone 3 (H3) resulting in inhibition of gene transcription within the euchromatic regions of chromosomes. Studies linking the activity of SetDB1 and H3 methylation can elucidate the mechanisms of tumorigenesis in various types of cancers.

UniProt

Q15047

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

96 ReactionsCatalog #ComponentAmountStorage51055SetDB1 Human Enzyme*2 μg-80°C5212020 μM S-adenosylmethionine250 μl-80°C52140APrimary Antibody 1100 μl-80°C52130HSecondary HRP-labeled Antibody 110 μl-80°C521604x HMT assay buffer 13 ml-20°C52100Blocking Buffer 450 ml+4°C0.5 M DTT200 µl-20°CHRP chemiluminescent substrate A (transparent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°C96-well plate precoated with histone substrate+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.384 ReactionsCatalog #ComponentAmountStorage51055SetDB1 Human Enzyme*4 μg-80°C5212020 μM S-adenosylmethionine2 x 250 μl-80°C52140APrimary Antibody 12 x 100 μl-80°C52130HSecondary HRP-labeled Antibody 12 x 10 μl-80°C521604x HMT assay buffer 12 x 3 ml-20°C52100Blocking Buffer 42 x 50 ml+4°C0.5 M DTT200 µl-20°CHRP chemiluminescent substrate A (transparent bottle) 2 x 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 2 x 6 ml+4°C384-well plate precoated with histone substrate+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when materials are stored as directed.Avoid multiple freeze/thaw cycles.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected].