SetDB1 Chemiluminescent Assay Kit

SetDB1 (Histone-lysine N-methyltransferase SETDB1) is a methyltransferase that reversibly catalyzes the di and trimethylation of histone 3 (H3) resulting in inhibition of gene transcription within the euchromatic regions of chromosomes. Studies linking the activity of SetDB1 and H3 methylation can elucidate the mechanisms of tumorigenesis in various types of cancers.

96 ReactionsCatalog #ComponentAmountStorage51055SetDB1 Human Enzyme*2 μg-80°C5212020 μM S-adenosylmethionine250 μl-80°C52140APrimary Antibody 1100 μl-80°C52130HSecondary HRP-labeled Antibody 110 μl-80°C521604x HMT assay buffer 13 ml-20°C52100Blocking Buffer 450 ml+4°C0.5 M DTT200 µl-20°CHRP chemiluminescent substrate A (transparent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°C96-well plate precoated with histone substrate+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.384 ReactionsCatalog #ComponentAmountStorage51055SetDB1 Human Enzyme*4 μg-80°C5212020 μM S-adenosylmethionine2 x 250 μl-80°C52140APrimary Antibody 12 x 100 μl-80°C52130HSecondary HRP-labeled Antibody 12 x 10 μl-80°C521604x HMT assay buffer 12 x 3 ml-20°C52100Blocking Buffer 42 x 50 ml+4°C0.5 M DTT200 µl-20°CHRP chemiluminescent substrate A (transparent bottle) 2 x 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 2 x 6 ml+4°C384-well plate precoated with histone substrate+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.

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