GCN5 Chemiluminescent Assay Kit

GCN5 ((General control of amino acid synthesis, Yeast, Homolog) -like 2), also known as KAT2A or lysine acetyltransferase 2A, can function as acetyltransferase, glutaryltransferase, succinyltransferase or malonyltransferase, depending on the cellular context, and it functions as a transcription regulator. As part of the SAGA (SPT-ADA-GCN5 acetyltransferase) and ATAC (ADA2A containing complex) complexes it functions as a histone acetyltransferase (HAT) on core histones. SAGA is a 19-subunit complex made of 4 units, including GCN5, that can recruit transcription proteins and modify promoter-proximal chromatin, being also involved in DNA damage repair and signaling pathways. ATAC can serve as a co-factor for c-Jun in the transcription of JNK (Jun N-terminal kinase) target genes. In the context of the hematopoietic lineage, GCN5 regulates maturation of NKT (natural killer T) cells, differentiation of granulocytic cells and proliferation of T cells. Both SAGA and ATAC have been linked to cancer and seem to be essential for cancer cell survival. A deep understanding of the GCN5 functions, and ability to inhibit or downregulate it may prove beneficial for patients suffering cancer where SAGA and ATAC are essential for cancer cell survival.

Catalog #NameAmountStorage50074GCN5 (KAT2A), FLAG-Tag*5 µg-80°C0.1 mM Acetyl-CoA10 µl-20°C797082x HAT Assay Buffer10 ml-20°C52140UPrimary Antibody 2112 µl-80°C52131HSecondary HRP-Labeled Antibody 210 µl-80°C52100Blocking Buffer 450 ml+4°C0.5M DTT200 µl-20°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom TempWhite plate (or strips) pre-coated with Histone-Substrate1 plate+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.

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The GCN5 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. Avoid the use of strong acids and bases, ionic detergents and high salt concentrations.