GCN5 Chemiluminescent Assay Kit

The GCN5 Chemiluminescent Assay Kit is an ELISA (enzyme-linked immunosorbent assay) -based assay designed to measure the histone acetyltransferase activity of GCN5 ((General control of amino acid synthesis, Yeast, Homolog) -like 2) for screening and profiling applications. The GCN5 assay kit comes in a convenient 96-well format, with enough recombinant purified GCN5 enzyme (amino acids 362-837), acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection and blocking buffer for 96 enzyme reactions.Figure 1:Schematic representation of the mechanism behind GCN5 Chemiluminescent Assay Kit.GCN5 protein is added to a plate pre-coated with a histone substrate in the presence of acetyl-CoA. After washing, an anti-acetyl-lysine antibody is added, followed by a secondary HRP-antibody. Lastly, HRP substrate is added and the chemiluminescence signal generated can be measured. The signal is proportional to the acetyltransferase activity of GCN5Need us to run inhibitor screens or profile your compounds against GCN5? Check out ourAcetyltransferase Screening Services.This product has beencited 1 time.

Product Specifications

Background

GCN5 ((General control of amino acid synthesis, Yeast, Homolog) -like 2), also known as KAT2A or lysine acetyltransferase 2A, can function as acetyltransferase, glutaryltransferase, succinyltransferase or malonyltransferase, depending on the cellular context, and it functions as a transcription regulator. As part of the SAGA (SPT-ADA-GCN5 acetyltransferase) and ATAC (ADA2A containing complex) complexes it functions as a histone acetyltransferase (HAT) on core histones. SAGA is a 19-subunit complex made of 4 units, including GCN5, that can recruit transcription proteins and modify promoter-proximal chromatin, being also involved in DNA damage repair and signaling pathways. ATAC can serve as a co-factor for c-Jun in the transcription of JNK (Jun N-terminal kinase) target genes. In the context of the hematopoietic lineage, GCN5 regulates maturation of NKT (natural killer T) cells, differentiation of granulocytic cells and proliferation of T cells. Both SAGA and ATAC have been linked to cancer and seem to be essential for cancer cell survival. A deep understanding of the GCN5 functions, and ability to inhibit or downregulate it may prove beneficial for patients suffering cancer where SAGA and ATAC are essential for cancer cell survival.

UniProt

Q92830

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage50074GCN5 (KAT2A), FLAG-Tag*5 µg-80°C0.1 mM Acetyl-CoA10 µl-20°C797082x HAT Assay Buffer10 ml-20°C52140UPrimary Antibody 2112 µl-80°C52131HSecondary HRP-Labeled Antibody 210 µl-80°C52100Blocking Buffer 450 ml+4°C0.5M DTT200 µl-20°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom TempWhite plate (or strips) pre-coated with Histone-Substrate1 plate+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

Contraindications

The GCN5 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. Avoid the use of strong acids and bases, ionic detergents and high salt concentrations.