Anti-Hu CD96 APC

CD96 is a 160-240 kDa type I transmembrane glycoprotein with intracellular ITIM-like domain, however, without observed inhibitory function. This protein is involved in adhesive interactions of T and NK cells during the late phase of the immune response, and are expressed above all upon their activation. Strong interaction between CD96 and CD155 (poliovirus receptor) stimulates cytotoxicity of activated NK cells, which may play a role in NK cell recognition and killing of CD155-expressing tumor cells. CD96 is expressed on approximately 80% of T cell leukemias and 30% of acute myeloid leukemias and is being used for their immunophenotyping.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human NK92 cell line

Target Antigen

CD96

Clone

NK92.39

Applications

FC (QC tested)

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Eriksson EM, Keh CE, Deeks SG, Martin JN, Hecht FM, Nixon DF: Differential expression of CD96 surface molecule represents CD8⁺ T cells with dissimilar effector function during HIV-1 infection. PLoS One. 2012;7 (12) :e51696., URL: https://www.ncbi.nlm.nih.gov/pubmed/23272144, *Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M: Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155) . J Immunol. 2004 Apr 1;172 (7) :3994-8., URL: https://pubmed.ncbi.nlm.nih.gov/15034010/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody NK92.39 recognizes an extracellular epitope of CD96, a transmembrane glycoprotein expressed at low levels on resting, and at high levels on activated T and NK cells, as well as on many T cell leukemias.