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Spike S1 RBD (B.1.1.7; Alpha Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) HiP™ Recombinant

Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. This protein corresponds to SARS-CoV-2 Variant B.1.1.7, also known as variant Alpha originally identified in the United Kingdom, and contains mutation N501Y. It was constructed with a C-terminal Avi-Tag™ followed by a His-tag (6xHis) . The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.HiP™indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

Product Specifications

UniProt

P0DTC2

Tag

C-terminal Avi-His-tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.For more information on enzymatic biotinylation, please see ourTech Note.

Purity

≥ 90%

Format

Aqueous buffer solution

Shipping Conditions

-80°C

Storage Conditions

At least 6 months at -80°C.

Calculated Molecular Weight

28 kDa + glycans

Formulation

8 mM phosphate pH 7.4,110 mM NaCl, 2.2 mM KCl, 20% glycerol

Aggregation

< 10%

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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