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Spike Trimer (S1+S2) (B.1.429, Epsilon Variant) His-Tag (SARS-CoV-2) Recombinant

Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.429, also known as variant Epsilon, originally identified in California (USA), and contains mutations W152C, L452R, D614G.  The construct also contains mutations 682RRAR685>A, K986P and V987P, and a T4 trimerization domain followed by a His-tag (6xHis) in C-terminal. The recombinant protein was affinity purified.

Product Specifications

UniProt

P0DTC2

Tag

C-terminal His-tag

Purity

85%

Format

Aqueous buffer solution

Shipping Conditions

-80°C

Storage Conditions

At least 6 months at -80°C.

Calculated Molecular Weight

139 kDa + glycans

Formulation

8 mM phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, 20% glycerol

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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