Spike Trimer (S1+S2), His-tag, Eu-labeled (SARS-CoV-2) Recombinant
Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein contains mutations 682RRAR685>A, K986P and V987P. The construct also contains a C-terminal His tag (6xHis) . The recombinant protein was affinity purified and labeled with trFluor-Eu.
Product Specifications
Tag
C-terminal His-tag
Label
TrFluor™ Eu (Ex/Em = 346nm / 617 nm) is a fluorophore with large Stokes shifts and an extremely long emission half-live. It is ideal for applications that require high sensitivity and for time-resolved fluorometry because a delay between excitation and the detection of emission minimizes interference. EU-labeled probes are characterized by a relatively high stability and are often insensitive to quenching. trFluor™ Eu is covalently and randomly conjugated to the target protein.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Purity
≥ 90%
Shipping Conditions
-80°C
Storage Conditions
At least 6 months at -80°C.
Calculated Molecular Weight
139 kDa + glycans
Formulation
40 mM Tris, pH 8.0,110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole and 20% glycerol.
Glycosylation
This protein runs at higher MW by SDS-PAGE due to glycosylation.
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