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KRAS (G12D), Isoform A, His-Tag Recombinant

Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end) . In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308) . The protein also contains mutation of interest G12D. The protein was affinity purified.

Product Specifications

UniProt

P01116

Tag

N-terminal His-tag

Purity

≥ 90%

Format

Aqueous buffer solution.

Shipping Conditions

-80°C

Storage Conditions

At least 6 months at -80°C.

Calculated Molecular Weight

23 kDa

Formulation

20 mM HEPES, pH 7.4,150 mM NaCl, 10% Glycerol, and 1 mM DTT

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