PARP15 Chemiluminescent Assay Kit

The PARP15 Chemiluminescent Assay Kit is designed to measure the activity of PARP15 (poly- (ADP-ribose) polymerase 15) for screening and profiling applications. The PARP15 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP15 enzyme (amino acids 221-444), reaction substrates, and PARP assay buffer for 100 enzyme reactions.Figure 1. PARP15 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP15 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP15 activity.Need us to run inhibitor screens or profile your compounds against PARP15? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.

Product Specifications

Background

PARP15, also known as poly- (ADP-ribose) polymerase 15 or ARTD7 (diphteria toxin like ADP-ribosyl transferase 7), is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP15 is involved in telomere maintenance, stress response, transcription and immune signaling. Little is known about PARP15, although some inhibitors have been identified. A deeper understanding of the function of this protein in health and disease will benefit the overall oncology field and may bring to light new treatment options.

UniProt

Q460N3

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Format

Catalog #NameAmountStorage80517PARP15, GST-Tag*1.5 µg-80°C520295x Histone Mixture1 ml-80°C78371PARP Substrate Mixture 2250 µl-80°C8060210x PARP Assay Buffer1 ml-20°C79743Blocking Buffer 325 ml+4°C0.5 M DTT200 µl-20°C80611Streptavidin-HRP100 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7983796-well module plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]