PARP11 Chemiluminescent Assay Kit

The PARP11 Chemiluminescent Assay Kit is designed to measure the activity of PARP11 (poly- (ADP-ribose) polymerase 11) for screening and profiling applications. The PARP11 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP11 enzyme (amino acids 8-338), reaction substrate, and PARP assay buffer for 100 enzyme reactions.Figure 1. PARP11 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP11 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP11 activity.Need us to run inhibitor screens or profile your compounds against PARP11? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.This product has beencited 2 times.

Product Specifications

Background

PARP11, also known as poly- (ADP-ribose) polymerase 11 or ARTD11 (ADP-Ribosyltransferase Diphtheria Toxin-Like 11), is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP11 is involved in the regulation of IFN-I (interferon I) receptor, IFNAR1, by performing MARylation on β-TrCP (β-transducin repeat-containing protein), which acts as an E3 ligase and ubiquitinates IFNAR1. The downregulation of IFNAR1 in CTLs (cytotoxic T lymphocytes) occurs when an immunosuppressive TME (tumor microenvironment) is present, in a process mediated by PARP11. Ablation of PARP11 can counteract this phenomenon, and the use of CTLs with a CAR (chimeric antigen receptor) that was engineered to inactivate PARP11 resulted in a higher efficacy in solid tumors. Targeting PARP11 genetically or pharmacologically may thus provide new therapeutic avenues in the treatment of solid tumors.

UniProt

Q9NR21

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage80511PARP11, GST-Tag, His-Tag*32 µg-80°C520295x Histone Mixture1 ml-80°C78371PARP Substrate Mixture 24 x 250 µl-80°C8060210x PARP Assay Buffer1 ml-20°C79743Blocking Buffer 325 ml+4°C0.5 M DTT200 µl-20°C80611Streptavidin-HRP100 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7983796-well module plateRoom Temp*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]