PARP10 Chemiluminescent Assay Kit

The PARP10 Chemiluminescent Assay Kit is designed to measure the activity of PARP10 (poly- (ADP-ribose) polymerase 10) for screening and profiling applications. The PARP10 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP10 enzyme, reaction substrates, and PARP assay buffer for 100 enzyme reactions.Figure 1. PARP10 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP10 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to PARP10 activity.Need us to run inhibitor screens or profile your compounds against PARP10? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.This product has beencited 1 time.

Product Specifications

Background

PARP10, also known as poly- (ADP-ribose) polymerase 10, NAD+ADP-ribosyltransferase 10 and ARTD10, is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP10 is found both in the cytosol and the nucleus, having c-myc, histones, PCNA (proliferating cell nuclear antigen), GSK3β (gyclogen synthase kinase-3 beta) and other proteins as ligands. It is involved in metabolism, with lower levels of PARP10 linking to lower fatty acid oxidation, DNA damage and repair in response to hydroxyurea and UV light and maintenance of genome stability. PARP10 has also been linked to cancer, acting as a tumor suppressor by negatively regulating Aurora A and thus negatively regulating G2/M transition during cell cycle. Inhibition of PARP10 presents itself as a potential new tool in cancer therapy.

UniProt

Q53GL7

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage80510PARP10*2 µg-80°C520295x Histone Mixture1 ml-80°C78371PARP Substrate Mixture 22 x 250 µl-80°C8060210x PARP Assay Buffer1 ml-20°C79743Blocking Buffer 325 ml+4°C0.5 M DTT200 µl-20°C80611Streptavidin-HRP100 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7983796-well module plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]