PARP6 Chemiluminescent Assay Kit

The PARP6 Chemiluminescent Assay Kit is designed to measure the activity of PARP6 (poly- (ADP-ribose) polymerase 6) for screening and profiling applications. The PARP6 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP6 enzyme, reaction substrates, and PARP assay buffer for 100 enzyme reactions.Figure 1. PARP6 Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP6 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP6 activity.Need us to run inhibitor screens or profile your compounds against PARP6? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.This product has beencited 2 times.

Product Specifications

Background

PARP6, also known as poly- (ADP-ribose) polymerase 6 or NAD+ADP-ribosyltransferase 6, is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP6 is involved in brain development, controlling dendrite morphogenesis, and it may be involved in disorders such as autism and Rett’s syndrome. In the absence of PARP6, DNA repair is less efficient, and cytotoxicity is potentiated when DNA damaging insults occur. PARP6 correlates with poor prognosis in breast, pancreatic and other cancer types. PARP6 can monoribosylate Chk1 (checkpoint kinase 1) and inactivate mitotic proteins, indicating a role of PARP6 in mitosis. It was also proposed that PARP6 can inhibit hepatocellular carcinoma by acting on the XRCC6 (X-ray repair cross complementing 6) /Wnt/β-catenin pathway. Use of AZ0108, a PARP inhibitor, resulted in MPS (multipolar spindle) and apoptosisin vitroandin vivo. A deeper understanding of PARP6 functions is required, and the development of molecules targeting PARP6 is a promising new cancer therapy tool.

UniProt

Q2NL67

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage80506PARP6, GST-Tag*25 µg-80°C520295x Histone Mixture1 ml-80°C78371PARP Substrate Mixture 24 x 250 µl-80°C8060210x PARP Assay Buffer1 ml-20°C79743Blocking Buffer 325 ml+4°C0.5 M DTT200 µl-20°C80611Streptavidin-HRP100 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7983796-well module plateRoom Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]