DPP9 Assay Kit

Dipeptidyl peptidase-9 (DPP9) is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP9 exhibits similar activity to that of DPP4 but unlike DPP4, DPP9 is not membrane bound. DPP9 activity has been linked to several diseases, including type-2 diabetes, obesity and cancer. TheFluorogenic DPP9 Assay Kitis designed to measure DPP9 activity using purified DPP9 for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is required for DPP9 reactions. The fluorogenic substrate is incubated with a sample containing DPP9 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against DPP9? Check out ourProtease Screening Services.This product has beencited 7 times.

Product Specifications

UniProt

Q86TI2

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage80090DPP9 human recombinant enzyme10 µg-80°CAvoidfreeze/thawcycles!80300DPP assay buffer10 ml-20°C80305Fluorogenic DPP substrate 1 in DMSO (0.5 mM) 100 µl-80°CAMC Fluorescent standard (50 µM) 500 µl-20°C79685black, low binding NUNC blackmicrotiter plate1 plateRoomtemp.

Shipping Conditions

-80°C

Storage Conditions

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Notes

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.