DPP4 Assay Kit

The Fluorogenic DPP4 Assay Kit is designed to measure DPP4 activity using purified DPP4 for screening and profiling applications. The key to the Fluorogenic DPP4 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP4 reactions; no time-consuming washing steps are required. The DPP fluorometric substrate is incubated with a sample containing DPP4 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against DPP4? Check out ourProtease Screening Services.This product has beencited 3 times.

Product Specifications

Background

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP4 plays a key role in glucose metabolism, immune regulation, signal transduction and apoptosis.

UniProt

P27487

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage80040DPP4 human recombinant enzyme1 µg-80°CAvoidfreeze/thawcycles!80300DPP assay buffer10 ml-20°C80305Fluorogenic DPP substrate 1 in DMSO (0.5 mM) 100 µl-80°CAMC Fluorescent standard (50 µM) 500 µl-20°C79685black, low binding NUNC blackmicrotiter plate1 plateRoomtemp.

Shipping Conditions

-80°C

Storage Conditions

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Notes

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.