Bald Lentiviral Pseudovirion (Luc-eGFP Dual Reporter)
The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) as the reporters, driven by a CMV promoter (Figure 1) . The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.Figure 1. Schematic of the Luciferase-P2A-eGFP Reporter in Bald Lentiviral PseudovirionThis product has beencited 2 times.
Product Specifications
Format
Since the virus is lacking the envelope glycoproteins and cannot transduce target cells, functional titer of this product cannot be determined. Based on p24 values, the approximate number of lentiviral particles (LP) of this product is ~109LP/ml.
Shipping Conditions
-80°C
Storage Conditions
Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.
Notes
BiosafetyNone of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Formulation
The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
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