Wee1 Kinase Assay Kit

Wee1 (also known as WEE1 G2 checkpoint kinase) belongs to the serine-threonine kinase family of proteins. Wee1 plays a crucial role in regulating G2/M transition through phosphorylation of cyclin dependent kinase (CDKs), allowing DNA damage to be repaired. that is overexpressed in many types of cancer such as luminal and HER2-positive breast cancer, hepatocellular carcinomas, and glioblastomas. Classical cancer therapies, such as chemotherapy and radiotherapy, induce DNA damage, and cells trigger the checkpoint in cell cycle, such as Wee1. This can lead to cancer cell resistance. Inhibiting Wee1 prevents cells from repairing DNA damage due to unchecked replication, suggesting that combination therapeutics with DNA damaging reagents and Wee1 inhibitors may be effective against cancer.

Catalog #NameAmountStorage40412Wee1, GST-Th-Tag*15 μg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP100 µl-20°C101574Wee1 Substrate (5 mg/ml) 200 µl-80°C79696White 96-well plate1RoomTemp.*The concentration of the protein is lot-specific and will be indicated on the tube